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Title   대장종양 생성에 관한 DMH 유발 종양모형의 적용성 판정 ( Applicability of DMH - Induced Colorectal Tumor Model in Respect to Carcinogenesis )
Publicationinfo   1992 Jan; 024(02): 239-249.
Key_word   DMH, Colorectal tumors, Applicability
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Abstract   DMH-induced colorectai tumor model has generally been accepted that the histopathological and biochemical characteristics were quite similar to the human colorectal tumors, but the sequences and biological traits were still controversiaL DMH-induced rat model was analysed by nuclear DNA analyses through flow cytometry as well as histopathology through light micros- copy and transmission electron microscopy, measurement of serum carcinoembryonic antigen, and tumorigenicity. 2 cases of colonic adenocarcinoma occurred in the low-dose(180mg/kg B. wt) of DMH, whereas 3 cases of colonic adenoma, 2 cases of colonic adenocarcinoma, 3 cases of duodenal adenocarcinoma, and a case of metastatic omental adenocarcinoma occurred in the high-dose(360mg/kg B.wt) of DMH on the 23rd week after DMH-treatment. 2 cases of adenomas and 3 cases of adenocarcinomas showed infiltrating growth into the lymphoid folli- cles or lymphoid hyperplasias without tumors of overlying mucosa#. The electron microscopic features of DHH-treated mucosae did not show the findings of hyperplasia or dysplasia. DNA indices were all diplaid, and proliferative indices did not show significant differences among tumors, transitional mucosae, and normal control mucosae. There were also no significant differencece betweeen serum carcinoembryonic antigen level of tumor and non-tumor group.as well as DMH-treated and control group. The anchorage-dependent culture of adenoma and adenocarcinoma were possible, but they grew very slowly without tumorigenicity of xenograft in the SCID mice. These results might be assumed that tumors of DMH-induced colorectal tu- mors developed from the field changes of susceptible mucosae and the paracrine factors in the submucosal lymphoid follicles could affect the carcinogenesis. In conclusion, DMH-treated co- lorectal tumors were quite different from human colorectal tumors in respect to the sequence and biological activity, and the applicability should be considered according to the purposes of experiment.
저 자   김진천(Jin Cheon Kim),공경엽(Gyeong Yeop Gong),박규연(Kyoo Yeon Park),김병식(Byung Sik Kim),박건춘(Kun Choon Park),이인철(In Chul Lee)